Recombinant Proteins from Plants : Production and Isolation of Clinically Useful Compounds

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Recombinant Proteins from Plants is one of the most exciting and fastest developing areas in biology. The latest molecular techniques are being applied to the exploitation of plants as novel expression systems for the production and overproduction of heterologous and native proteins. Transgenic plant technology is currently used in three broad areas: the expression of recombinant proteins to improve crop quality by increasing disease/pest resistance or increasing tolerance to stress, optimizing plant productivity and yield by the genetic manipulation of metabolic pathways, and the large-scale costeffective production of recombinant proteins for use as specialist industrial or therapeutic biomolecules. The intention of Recombinant Proteins from Plants is to provide comprehensive and detailed protocols covering all the latest molecular approaches. Because the production oftransgenic plants has become routine in many laboratories, coverage is also given to some of the more "classical" approaches to the separation, analysis, and characterization of recombinant proteins. The book also includes areas of research that we believe will become increasingly important in the near future: efficient transformation of monocots with Agrobacterium tumifaciens, optimizing the stability of recombinant proteins, and a section highlighting the immunotherapeutic potential of plant-expressed proteins. The contents bring together a broad range of knowledge and expertise from around the world; each chapter is written by research workers with hands-on experience of the protocols they describe. The book opens with chapters covering the introduction of transgenes into monocot and dicot species, and includes the expression and characterization of reporter genes and the application of recombinant proteins in crop protection. This is followed by sections that describe the expression of cytokines, antibodies/antibody fragments, and use of recombinant plant viral particles as vaccines. The later part of the book deals with the expression of proteins by plant suspension culture/protoplasts, explores the latest strategies to increase the yield of recombinant proteins by controlling the cellular location of protein folding, and the current protocols available for the molecular manipulation of photosynthetic pathways. The final chapters of the book outline techniques specifically designed to quantify and characterize plant-derived proteins and include: glycosylation state, ELISA, and two-dimensional electrophoresis.

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Informasi Detail

Judul Seri

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No. Panggil

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Penerbit

Canada : Gramedia Pustaka Utama., 1998

Deskripsi Fisik

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Bahasa

English

ISBN/ISSN

0-89603-390-2

Klasifikasi

NONE

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

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Info Detail Spesifik

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Pernyataan Tanggungjawab

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Versi lain/terkait

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