PLANT CYTOGENETICS Second Edition
Plant cytogenetics is progressing at an extremely rapid pace since the publication of the rst edition of Plant Cytogenetics by CRC Press in 1993. The time is ripe to update this book (second edition) in an encyclopedic fashion that includes two new chapters on the mode of reproduction in plants and transgenic crops. The introduction ows directly into the stream of classical and modern cytological techniques after a brief exposure to Mendelism and major chronological discoveries in the science of genetics. The handling of plant chromosomes assembled in Chapter 2 has undergone monumental progress. Precise identication and nomenclature of plant chromosomes from aceto-carmine and Feulgen staining techniques have progressed to Giemsa banding, uorescence in situ hybridization, and sorting and karyotyping of chromosomes by ow cytometry. Several protocols for determining nuclear DNA content by ow cytometry have been formulated and perfected. Cell division is a complex and mysterious phenomenon, and it has not been completely understood. Chapter 3 (cell division mitosis and meiosis) and Chapter 4 (genetic control of meiosis) present mainly classical descriptions of mitosis and meiosis in the higher plants. The mode of reproduction (sexual, asexual, and sex chromosomes) in the higher plants is described in Chapter 5. Chapter 6 includes chromosome nomenclature based on kinetochore position on chromosomes, karyotyping by aceto-carmine, and Giemsa C- and N-banding methods. Chromosome numbers of some economically important plants are presented in one of the appendices (6-I.). Chapter 7 discusses chromosomal aberrations (structural and numerical chromosome changes). Utilization of primary, secondary, and tertiary gene pools for crop improvement depends upon the comprehension of genomic relationships between the cultigen and allied species and genera. Genomic relationship established based on cytogenetics and veried by biochemical and molecular methods is presented in Chapter 8. The cause of morphological aberrations in transformants from cell and tissue culture is described in Chapter 9. The dazzling progress achieved since 1986 in producing genetically modied organisms (GMO) by private and public institutions is briey elaborated in Chapter 10. I have provided an adequate literature citation; it covers publications from ancient to the present. The effectiveness of the second edition was improved by including an extensive glossary. The appendices include recipes for rescuing immature embryos and karyotyping by ow cytometry and the current linkage map of barley.
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